Coding

Part:BBa_K519010:Design

Designed by: Kotone Miyake   Group: iGEM11_Tokyo-NoKoGen   (2011-09-30)

SmtA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 93
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 93
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 93
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was cloned from Synechococcus sp. PCC7942, using primers to add EcoRI, XbaI and SpeI at the ends.


Source

Synechococcus sp. PCC7942

References

Construction of a marine cyanobacterial strain with increased heavy metal ion tolerance by introducing exogenic metallothionein gene, Koji Sode et al., J Mar Biotechnol (1998), 6:174-177


edited by SCAU-China [1] Blindauer C A , Harrison M D , Parkinson J A , et al. A metallothionein containing a zinc finger within a four-metal cluster protects a bacterium from zinc toxicity[J]. Proceedings of the National Academy of Sciences, 2001, 98(17):9593-9598. [2] Blindauer, C.A., Harrison, M.D., Robinson, A.K., Parkinson, J.A., Bowness, P.W., Sadler, P.J. and Robinson, N.J. (2002), Multiple bacteria encode metallothioneins and SmtA‐like zinc fingers. Molecular Microbiology, 45: 1421-1432. doi:10.1046/j.1365-2958.2002.03109.x